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Old 10-09-2013, 03:31 AM   #15
Location: Milan

Join Date: May 2013
Posts: 40


I need some information about the exomes.bed file which you are using. I see that most of the pipelines say that they generate it from the UCSC genome browser to restrict the output to exonic sequences, is there any specific cut off that must be used to generate this bed file for the hg19 ref gene or a simple selection will do? or do i have to specify some extra bp addition for both end to catch the splice sites? Please let me know. On what criteria is the bed file selected or it is just a representation bed file of the whole ref genome restricted only to the exonic regions? Please let me know
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