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Old 08-27-2014, 06:38 PM   #2
Brian Bushnell
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Location: Walnut Creek, CA

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The HiSeq platform can generate reads of various lengths, strand-specific or non-strand-specific, paired or unpaired; 1x50 and 2x50 runs are both possible. It might help if you posted the first two reads (first 8 lines) of the fastq file here, but I'm not really sure what your question is.
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