SEQanswers - View Single Post - Ribo-zero bacteria vanishing RNA

ScienceGrrl · 09-16-2015, 10:59 AM

I had the opposite problem in my previous position of too much sample at the end b/c of failure to remove rRNA. I tested my depletion using Illumina's suggestion of running qPCR assay using GAPDH and 18S to see the amount of material before and after depletion. If your non-rRNA species are low in the pre depletion, you may be getting all you can.

09-16-2015, 10:59 AM	#3
ScienceGrrl Junior Member Location: Indianapolis, IN Join Date: Jul 2015 Posts: 8	I had the opposite problem in my previous position of too much sample at the end b/c of failure to remove rRNA. I tested my depletion using Illumina's suggestion of running qPCR assay using GAPDH and 18S to see the amount of material before and after depletion. If your non-rRNA species are low in the pre depletion, you may be getting all you can.