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Old 03-14-2017, 01:26 AM   #1
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Location: Cape Town, South Africa

Join Date: Jan 2017
Posts: 6
Default Reason for POOR per tile sequence quality

We recently performed a sequencing run on the MiSeq sequencer using a V3 (600 cycle kit).

With regards to the per tile quality plot (obtained when using the FastQC analysis software) for one of our samples, it seems as though there is a large area that is flagged in red for R2, indicating that those tiles had worse qualities.

I was just wondering on possible reasons why this would happen and how it could be prevented in future runs?

Attached Images
File Type: jpg R1.jpg (60.1 KB, 40 views)
File Type: jpg R2.jpg (71.7 KB, 25 views)
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