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Old 10-18-2010, 12:58 AM   #9
simonandrews
Simon Andrews
 
Location: Babraham Inst, Cambridge, UK

Join Date: May 2009
Posts: 871
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Quote:
Originally Posted by Bio.X2Y View Post
Hi Simon, thanks for this. Out of interest, when you say you do 10% of a lane, how is this done? I'm not very familiar with the sequencing procedure itself, but I imagined it was an all-or-nothing, and you couldn't back out after 10%.
We still run a control lane on each flowcell because of the nature of many of our libraries. What we can therefore do is to mix in 10% of another sample alongside the PhiX and then extract out everything which doesn't map to PhiX at the end of the run to get a small scale view of the other library.
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