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Old 09-06-2011, 08:24 PM   #1
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Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246
Default TruSeq DNA adapters in RNA-seq prep... concentration?

Hi Everyone,

I'm in a situation whereby I need to use two extra adapters than the 6 adapters that are currently shipped with each set of the TruSeq RNA-seq prep kit and at the moment we only have one set. We have both sets of adapters for the TruSeq Genomic DNA prep kit (i.e. all 12 adapters).

I know the Genomic DNA and RNA-seq adapters are the same, but the concentration is different. Has anyone used the adapters from the DNA kit in the RNA sample prep? I think it used to be a 10 fold dilution of adapters using the 'old', non TruSeq kits.


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