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Old 09-26-2011, 12:35 PM   #1
OnUrMark
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Location: Oregon

Join Date: Jan 2011
Posts: 24
Default TruSeq RNA initial concentration and Ampure beads

Greetings!

I am getting ready to use the TruSeq RNA kit for the first time. I noticed the concentration of Ampure beads to sample decreases as you progress through the steps to prepare an RNASeq sample. I assume that as the bead concentration to DNA decreases, the maximum length of fragment washed away will increase.

Ex: 1.8:1 bead: DNA removes anything less than 100bp. Then perhaps 1:1 bead: DNA would remove anything less than 200bp.

1) Is this a correct assumption?
2) Has anyone found that varying the initial RNA concentration affects the maximum length of fragment washed away and results in a different final fragment length?

Thanks in advance for your help and sharing your knowledge on this preparation technique.
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