SEQanswers - View Single Post - TruSeq RNA initial concentration and Ampure beads

RNAseqer · 09-27-2011, 05:31 AM

According to what I've read anything greater than a 1x ratio will recover everything <~100. Its only when you go less than 1x do you start being able to size select, ie. .5X or .75X. I'd be interested in the experience of others too.

09-27-2011, 05:31 AM	#2
RNAseqer Member Location: London Join Date: Sep 2010 Posts: 22	According to what I've read anything greater than a 1x ratio will recover everything <~100. Its only when you go less than 1x do you start being able to size select, ie. .5X or .75X. I'd be interested in the experience of others too.