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Old 04-03-2012, 11:35 PM   #5
oxydeepu
Member
 
Location: bangalore,india

Join Date: Jul 2011
Posts: 41
Default Similiar sort of problem.

Hi all,

I am getting this error

Traceback (most recent call last):
File "/home/deepak/bioTools/tophat-1.3.1.Linux_x86_64//tophat", line 2607, in ?
sys.exit(main())
File "/home/deepak/bioTools/tophat-1.3.1.Linux_x86_64//tophat", line 2504, in main
params.read_params = check_reads_format(params, reads_list)
File "/home/deepak/bioTools/tophat-1.3.1.Linux_x86_64//tophat", line 1234, in check_reads_format
zf = ZReader(f_name, params.system_params)
File "/home/deepak/bioTools/tophat-1.3.1.Linux_x86_64//tophat", line 1185, in __init__
self.file=open(filename)
IOError: [Errno 2] No such file or directory: '-o'


while running tophat

tophat -r 200 -i 50 -I 250000 /home/deepak/bowtie-0.12.5/indexes/hs_genome 37_NoIndex_L001_R1_001.fastq -o tophat_new &

Please help me with this error.

Thanking you in advance.

Regards
Deepak
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