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Old 04-05-2012, 02:56 AM   #1
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Location: London

Join Date: Nov 2011
Posts: 3
Default Amplicon sequencing - gel cut purification

Hi all,

For our next 454 amplicon sequencing run we are thinking to do a gel cut purification of our amplicons in order to eliminate small fragments e.g primer dimers (they may be getting through our libraries unnoticed as we have quite a large percentage of reads failing on 'short quality').

Can anyone recommend an effective kit/method for this which has given good results? Also, as I will pool multiple samples together, could I do this gel cut on the entire pooled library or would it need to be the individual amplicons (it is a 16S library so all amplicons should be the same size).

Any advice would be hugely appreciated!

Many thanks,
JKistler is offline   Reply With Quote