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Old 04-10-2012, 06:11 AM   #5
Junior Member
Location: London

Join Date: Nov 2011
Posts: 3

Thanks to all for your advice. I'll go with a gel cut after pooling and optimise PCR as best as possible to reduce primer dimers. Any improvement, would be good. That's worrying to hear that the primer dimers can anneal back to full length strands though.
JKistler is offline   Reply With Quote