Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • De novo assembly of a small region after using PINDEL?

    Hello again,
    I have just recently got the program PINDEL to work on my reads, and it identified a large deletion (9kb) in my region of interest (gene family, roughly 40kb). What I would really love, is to take all the reads from PINDEL, and my original alignment from that particular area, and re-align them without the reference, to see if it can make sense of the region. Has anyone had any luck doing such a thing? If so, can you point me in the right direction?
    Thanks so much
    A

    /I've also already designed primers to test if this deletion is legit, so that's obviously a first step.

  • #2
    Try Tigra-SV


    An international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms

    Comment


    • #3
      Tigra looks interesting, but from what I can tell that version is no longer being developed, and the original author now has version 0.3.9 here:



      Also, neither of these will compile with samtools 0.1.19, but 0.1.18 works.
      For the benefit of Google searchers, the error message with 0.1.19 is
      /usr/lib/samtools/libbam.a(bgzf.o): In function `bgzf_mt':
      .../samtools-0.1.19/bgzf.c:445: undefined reference to `pthread_create'
      /usr/lib/samtools/libbam.a(bgzf.o): In function `mt_destroy':
      .../samtools-0.1.19/bgzf.c:458: undefined reference to `pthread_join'

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      26 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      29 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      25 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Working...
      X