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  • Error running blastdbaliastool

    SOLVED: The makedb tool was not creating index files. I directly downloaded the database from NCBI and it is working.

    Hi,
    I am running command line blastplus, and need to blast against bacteria. I have downloaded databases nr and nt, but need to create a subset for faster searching. I have followed the method in

    where I have downloaded a list of accession numbers for bacteria, and am trying to create a subset database based on this. I have tried the command

    blastdb_aliastool -gilist bacteria.gilist.txt -db nr -out nr_bacter -title nr_bacteria
    but I get an error
    "BLAST Database error: GI list specified but no ISAM file found for GI."

    Any idea what this is?
    Thanks so much
    Last edited by nupurgupta; 06-14-2012, 09:37 AM.

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