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Old 06-21-2013, 05:57 AM   #3
Susanne
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Location: Germany

Join Date: Aug 2009
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The HS chip is quite sensitive with regards to genomic DNA and overloading. So I would recommend not to load more than 50 ng/Ál, better around 10-15 ng/Ál if you want to quantify. There are several problematic aspects to it though - if your sample runs into the upper marker, your quantification will be biased, and if you load too much, the quantification will also be wrong.
Genomic DNA cannot enter the channels and it's possible that the chip doesn't run properly (if the gDNA is not degraded).
The 0.5 ng/Ál quoted for the 12000 chip is referring to this concentration in a single peak. You will not see this concentration in a smear sample (more like 5 ng/Ál required).
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