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Old 04-09-2017, 04:07 PM   #1
Location: Sweden

Join Date: Nov 2014
Posts: 18
Default Please help with assembly 2x250 bp with 350 insert

Hi folks,

I have got data generated from 2x250 bp with 350 bp insert for fungal genome assembly. I got very good results from SPAdes, but later discovered that SPAdes recommends an insert of 550-700 bp for 2x250 bp sequencing.

I have tried Abyss, Velvet and CLC so far, but they did not give as good results as SPAdes. Do you have opinion or suggestion what else I shall try? Or would it be just okay to use SPAdes?

Thanks in advance and have a great weekend!
Vinn is offline   Reply With Quote