Hi all,
newbie here. We are using the Nextera kit to prepare libraries but the local NGS facility doesn't support it unless we fulfill 8 lanes due to cost. I'm going through the Hiseq 2000 manual trying to figure out the difference in sequencing procedure between Nextera and Truseq, but it seems they just require different primers, which look pretty cheap...? I'm wondering if anyone can shed some light on this? Also, we are looking for sequencing facilities who support small number of submission of Nextera libraries... Thank you!
newbie here. We are using the Nextera kit to prepare libraries but the local NGS facility doesn't support it unless we fulfill 8 lanes due to cost. I'm going through the Hiseq 2000 manual trying to figure out the difference in sequencing procedure between Nextera and Truseq, but it seems they just require different primers, which look pretty cheap...? I'm wondering if anyone can shed some light on this? Also, we are looking for sequencing facilities who support small number of submission of Nextera libraries... Thank you!
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