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Old 01-30-2015, 04:03 AM   #1
Location: Europe

Join Date: Mar 2014
Posts: 42
Default Assembled contigs vs short reads

I have recently finished assembling some metagenome sequences and after assigning function to my contigs I see that most genes belong to three specific types of microorganisms. I also submitted the unassembled short reads to MG-RAST to get an overview of functional genes. However when I look through the MG-RAST results the genes that are most abundant are not necessarily the same ones that dominate the assembled contigs. I was wondering why the two types of information wouldn't match?
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