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Old 02-27-2015, 04:29 PM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

You can scale down Nextera. I would pool the amplicons from each sample (it sounds like that is allowed, just not pooling the species), fragment a few ng worth from each sample and PCR with a unique dual index barcode, and then sequence by either slipping the samples in with a friend's run or finding the cheapest possible "lane" like a low NextSeq.
Providing nextRAD genotyping and PacBio sequencing services.
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