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Old 09-26-2017, 12:14 PM   #6
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
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Read 1 begins with the RT primer. It contains the cell barcode, UMI, and oligo(dT), which primes 1st strand synthesis from the mRNA's poly(A) tail. Until you get past the poly(A), which can be long, there's no useful information to be gained from sequencing past the UMI.
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