View Single Post
Old 03-14-2018, 08:49 PM   #5
sbarberan
Member
 
Location: Santa Cruz, CA

Join Date: Feb 2017
Posts: 17
Default

Hi Phillip,

Really interesting points about purification.

You mention that using the RNA chip allows you to see primers/dimers even if they are annealed to full length library molecules; and that if this is the case you can purify them with AMPure.

My question is, if they are annealed to full length library molecules how come AMPure is able to remove them? AMPure will not denature the libraries so if they are annealed they will be co-purified, right? am I missing something?

Cheers,
sergio
sbarberan is offline   Reply With Quote