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Old 05-13-2018, 11:04 PM   #3
filipeuit
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Location: Tromsų

Join Date: Apr 2018
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Quote:
Originally Posted by gabrieltw View Post
Check out

doi:10.1038/nprot.2018.019
Thank you very much, Gabriel. That is a very thorough protocol, that I was not aware existed.

EDIT: Do you think molarity differences between libraries will be an issue? If you run a TruSeq library prep protocol, and you start with e.g. 1 ug of sheared DNA per sample, you will end up with libraries that have different molarities. Before pooling libraries prior to sequencing, we usually QC through Picogreen/Qubit and qPCR so we can dilute and pool samples with the same (or similar) molarity. Would it be important to qPCR the samples prior to pooling before BP size selection?

Last edited by filipeuit; 05-14-2018 at 01:24 AM. Reason: Question pertinent to pooling of libraries
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