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Old 05-14-2018, 03:39 AM   #4
Location: Boston

Join Date: Mar 2013
Posts: 24

Look up the part that use limited primers to normalize libraries in the paper. After the limited primer normalization, most of the libraries should be around the same concentration. Basically steps are :

Library prep -> QC -> PCR enrichment with limited primer -> pooling -> size select -> pooled libraries loading.
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