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Old 07-19-2018, 07:35 PM   #3
LMA_2018
Junior Member
 
Location: Sri Lanka

Join Date: Jul 2018
Posts: 4
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Hi r.rosati,
Yes they do look like dimers. We run a fusion primer approach to amplify our target. The FAS did mention that there was a problem with our purification process which we tweaked with a different protocol to try and improve the output. I am attaching gel image of the libraries that we get normally.

Unfortunately we do not have a bioanalyzer at our facility so we had no option other than to run our purified libraries on a gel to see how our purification process was working (see images).

We are working on refining our library process which is no doubt contributing to the problem but our control library runs are also pretty bad, which is why we are trying to see if anyone else has come across this problem .
Attached Images
File Type: jpg Final Library.jpg (48.3 KB, 22 views)
File Type: jpg B43-65 gel images.jpg (94.3 KB, 15 views)
File Type: jpg Pool.jpg (6.8 KB, 10 views)
Attached Files
File Type: pdf UTF-8''Auto_user_S5XL-27-19718_400bp_S5_OT2_520_chip_run_plan_134_tn%20%281%29.pdf (515.5 KB, 4 views)
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