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Old 10-09-2018, 01:05 PM   #1
ntremblay
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Location: Montreal, Quebec, Canada

Join Date: Dec 2009
Posts: 30
Question AAAAAA.. TTTTTTT.. heterodimers

Good evening all,

I have prepared (ds)cDNA from viral RNA extracted from plasma that was subsequently sequenced on a MiSEQ following standard library preparation.

When I got the reads back, I only have TTTTTTTTTTTTTT or AAAAAAAAA sequences for 99.9% of my reads. Read have good phred score and are of the expected length (100 bp).

Any ideas about why I might get such a result?

Thanks!

Nicolas
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