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Old 10-16-2018, 06:55 AM   #5
ntremblay
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Location: Montreal, Quebec, Canada

Join Date: Dec 2009
Posts: 30
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Viral RNA was extracted from plasma samples using QIAamp Viral RNA Mini Kit (720uL of sample) using standard protocol.

Double stranded cDNA synthesis was done using Maxima H Minus Double-Stranded cDNA Synthesis Kit (random hexamers, Oligo (dT) and HCV specific pan genomic primers dA20) following standard protocol cycling conditions.

The dsCDNA was quantified by nanodrop and promega QuantiFluorŽ dsDNA System.

I will inquired how the cDNA was prep for sequencing.

Thanks,

Nicolas
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