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Old 10-16-2018, 11:32 PM   #6
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,231
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I do not know why oligo dT has been used in 1st strand synthesis. It is possible that dA20 oligo has hybridized to oligo T resulting in creating long stretches of T by joining that has been used as template by reverse transcriptase. Because of high primer concentration polyT artefact has been dominant species in the reactions and has ended up in final library.
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