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Old 07-22-2019, 01:13 PM   #2
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Location: US

Join Date: Dec 2010
Posts: 430

Sorry, I do not fully understand your description of the problem. In any case, it would be best to pool your libraries at a low percentage with other (best complex) libraries (that have differing barcodes) to get a look at data of these libraries.

I assume the problem is the poly-A tail added by the CATS protocol? The quality in this region will certainly be low if sequenced straight-up. On the other hand, you should not need data for these cycles of the read.
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