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Old 10-15-2019, 01:17 PM   #1
kgoglin
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Location: San Diego

Join Date: Dec 2014
Posts: 17
Angry qPCR standard variability with Kapa kit

We have been using Kapa's qPCR kit for Illumina library quantification for years. This year, it has given us great problems with inconsistent standards.

We have seen perfect triplicates one day and highly variable triplicates the next with the same standards. We store the standard aliquots at -20 to avoid freeze/thaws. We clean the hood prior to every run. We have tried different tubes, switching to lo bind for standard storage. We had all our pipets calibrated. We also have even changed qPCR instruments!

Has anyone else experienced this with Kapa's kit? Since we cannot find a solution, we will begin testing NEBNext's qPCR quant kit. Hopefully that will be the solution.
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