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Old 10-15-2019, 10:59 PM   #2
GSviral
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Location: UK

Join Date: Dec 2014
Posts: 37
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Hi kgoglin,

When I previously used the KAPA qPCR quantification method I also ran into the same problem from time to time. Sometimes triplicate standards could be very variable between runs even though I took all the precautions you mentioned in your post. Additionally, I used to run PhiX as a control on a KAPA run. Although I know the concentration of PhiX can be variable, sometimes I was getting absolutely wild results from a quantification run, completely out of the range I would expect the PhiX concentration to fall in. I never found out what the problem was.

Subsequently, my lab changed to NEB qPCR quantification and the last I heard it was performing really well for them so hopefully you will see some reliable results when you use it.
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