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If you want to know which sequences (transcripts/unigenes/protein) belong to which biological function/gene ontology term with Blast2GO you can use the "Combined Graph Function" and then export the graph as .txt file (from the side-panel). This gives you a spreadsheet with a list of all sequence IDs for each GO terms (one GO per row) for all GO levels and categories. This means direct and indirect functional annotations for MF, CC and BP.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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