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Old 02-27-2020, 08:01 AM   #4
kmcarr
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Location: USA, Midwest

Join Date: May 2008
Posts: 1,177
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mdegraaf,

You didn't say how many libraries you pooled. If it is more than 12 then it is inevitable that you have more than one library sharing a common i7 index sequence. i7 is the first of the two index sequenced or the ONLY index sequenced if you choose PE150+8+150. You can not use this format if any i7 index is repeated in your pool, you must then use PE150+8+8+150. Honestly I'd always choose the dual index sequencing format; what would be the downside to it?
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