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Old 03-25-2020, 10:17 AM   #2
ajthomas
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Location: Utah

Join Date: Mar 2010
Posts: 166
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It sounds like you want to do something similar to 16S rRNA sequencing. That is a well-established protocol. I don't know what your amplicon is, but you can probably get some pretty good guidance from the 16S protocols out there. You would just need to replace the 16S primer sequences with your own.
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