Dear All,
I'm working on some ancient blood samples(about 20yrs old), trying to do some metagenomic studies on microbiota. The issue is: most of the library prep products' average bp falling around 250 like what was attached here. Is this MiSeqable? Your ideas and suggestions are more than welcome!
Tons of thanks!
Yue
PS: the library was prepared through the Nextera XT kits, and the tagmentation time was 2:30 minutes, amplified by 15 cycles. The others were strictly following Nextera XT protocol.
PPS: DNAs were probably degraded, but I randomly bioanalyzed the DNAs, some of their average bp were truly very low, lower than 250, but we did get some DNAs between 180-1100 bp, nevertheless, the library prep products are the similar as attached.
I'm working on some ancient blood samples(about 20yrs old), trying to do some metagenomic studies on microbiota. The issue is: most of the library prep products' average bp falling around 250 like what was attached here. Is this MiSeqable? Your ideas and suggestions are more than welcome!
Tons of thanks!
Yue
PS: the library was prepared through the Nextera XT kits, and the tagmentation time was 2:30 minutes, amplified by 15 cycles. The others were strictly following Nextera XT protocol.
PPS: DNAs were probably degraded, but I randomly bioanalyzed the DNAs, some of their average bp were truly very low, lower than 250, but we did get some DNAs between 180-1100 bp, nevertheless, the library prep products are the similar as attached.
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