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Old 03-16-2014, 10:17 PM   #14
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Location: hk

Join Date: Oct 2012
Posts: 8

Hi, daantje, have you figured out the cause of low yield? I tried the kit recently and also encountered similar problem, only got less than 1ng amplified cDNA from 500pg input RNA provided in the kit after 14 cycles. I would really appreciate if anyone can offer some suggestions for this kit.

Originally Posted by daantje View Post
Hi Yes, would also be interested in hearing if there are tricks for the SMART kit. I tried and with a 10ng input of control RNA, I only get 500pg of amplified cDNA. Even though the size is correct and I am happy to get to this point, still my yield is much lower then suggested in the manual. I have not yet proceeded with library generation.
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