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Old 03-16-2014, 09:17 PM   #14
liaojinyue
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Location: hk

Join Date: Oct 2012
Posts: 8
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Hi, daantje, have you figured out the cause of low yield? I tried the kit recently and also encountered similar problem, only got less than 1ng amplified cDNA from 500pg input RNA provided in the kit after 14 cycles. I would really appreciate if anyone can offer some suggestions for this kit.

Quote:
Originally Posted by daantje View Post
Hi Yes, would also be interested in hearing if there are tricks for the SMART kit. I tried and with a 10ng input of control RNA, I only get 500pg of amplified cDNA. Even though the size is correct and I am happy to get to this point, still my yield is much lower then suggested in the manual. I have not yet proceeded with library generation.
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