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Old 12-16-2017, 11:04 PM   #254
mike137
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Location: Philadelphia

Join Date: Dec 2017
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Quote:
Originally Posted by SunPenguin View Post
Has anyone here looked at what the TSO would be doing in subsequent PCR reactions (i.e. could it cause TSO founded amplification)?
I remember reading a protocol a few years back where they inserted several dU in the TSO sequence, then came in with USER enzyme following RT to digest the TSO (both free and incorporated). Does anyone know what that paper was? I'd like to try that.

Bead purification will also remove the TSO, but like many in this thread, I'm not crazy about post-RT purification. Need every single molecule for my PCR!
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