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Old 06-08-2018, 07:10 AM   #258
ChristmasSunflower
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Location: US

Join Date: Mar 2009
Posts: 15
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Hello,

I recently used Smart_Seq2 protocol for single cell study. These single cell is manually picked up into 8-strip PCR tube. I designed all 3 primers TSO, Oligo-dT30VN, and ISPCR oligo with biotin modification in 5' side. I did a few of experimental samples, along with RNA positive control RNA 1ng, 100pg and 10pg, and water negative control. I did 18 PCR cycles for preamplification and 1:1 beads ratio for PCR cleanup. From Qubit reading, it's hard to say if protocol worked. I run them with Bioanalyzer. It seems results are positive. Can someone here with more experimental experience comment this result?
https://www.dropbox.com/s/htc0pp0f8r...Afile.jpg?dl=0

Thanks
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