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Old 10-11-2011, 11:00 AM   #1
Location: Brasil

Join Date: Jun 2011
Posts: 12
Default Exon Sequencing of Human Chromosome X: Im in serious trouble


My lab had discovered a locus in chromosome X that is involved with a kind of cortical malformation in a family with a pattern of X dominant inhiritance. We discovered this locus by linkage analysis. So we have performed exome enrichment of Ch X exome and second generation sequencing in a Illumina plataform. A paired end library was built (75 bp aproximatelly). Well ...there are a lot of duplicated genes and regions.... so bioinformatic analysis is not capable to say if region X is really X or Y ... because the homology is between 90 and 100% .... Thus my SNP calling is not reliable....
My supervisor told me to perform Sanger sequencing...but is almost impossible to pick primers in this region
There is other solution for my problem ?
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