SEQanswers - View Single Post - Exon Sequencing of Human Chromosome X: I´m in serious trouble

ulz_peter · 10-11-2011, 11:47 PM

Hi,

In case theduplicated regions are not too long, you could perform a long-range PCR (up to 25kb or so) and put the primers in regions with no duplication. This may serve as template for PCRs used for Sanger sequencing or may be directly sequenced (depending on the yield after LR-PCR).

Any other ideas? (Well you could do FlowSorting of Chromosomes, but that's not too easy)

10-11-2011, 11:47 PM	#2
ulz_peter Senior Member Location: Graz, Austria Join Date: Feb 2010 Posts: 219	Hi, In case theduplicated regions are not too long, you could perform a long-range PCR (up to 25kb or so) and put the primers in regions with no duplication. This may serve as template for PCRs used for Sanger sequencing or may be directly sequenced (depending on the yield after LR-PCR). Any other ideas? (Well you could do FlowSorting of Chromosomes, but that's not too easy)