View Single Post
Old 09-24-2013, 06:26 AM   #6
Olaf Blue
Member
 
Location: Madison, WI

Join Date: Nov 2010
Posts: 58
Default

Hi, the EpiGnome product uses the Zymo Gold or Zymo Lightning conversion kits; Zymo claims no bias and can be used with as little as 500 pg input genomic DNA. The DNA post-conversion is used to construct the libraries for sequencing on Illumina paired end flow cells. The PCR primers used for library amplification/enrichment are not methylated; methyalted primers could be used but from what we understand they're insanely expensive.
Olaf Blue is offline   Reply With Quote