View Single Post
Old 09-25-2013, 09:18 AM   #7
bbm
Member
 
Location: North Carolina

Join Date: Sep 2011
Posts: 36
Default

Quote:
Originally Posted by Olaf Blue View Post
Hi, the EpiGnome product uses the Zymo Gold or Zymo Lightning conversion kits; Zymo claims no bias and can be used with as little as 500 pg input genomic DNA. The DNA post-conversion is used to construct the libraries for sequencing on Illumina paired end flow cells. The PCR primers used for library amplification/enrichment are not methylated; methyalted primers could be used but from what we understand they're insanely expensive.
Hi Olaf Blue,
Thank you for helping me and all the infor. I think I'll try the epicentre kit. What software or tool do you use for sequence data analysis? Is the BS reads in one of the 4 forms (+fw, +rc, -fw, -rc) or just 2 forms ? Thanks again!
bbm is offline   Reply With Quote