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Old 09-25-2013, 01:42 PM   #9
anth
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Join Date: Jul 2011
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Quote:
Originally Posted by Olaf Blue View Post
Hi BBM, basically the way we do the data analysis is (1) to trim off the first 6 bases off of the 5'-end, followed by adapter trimming using "Trimomatic" (I am not a Bioinformatcian so I get this information from my Core facility). After adper sequences are trimmed, then the sequence analysis is performed using Bismark. If you have anything more specific, let me know.

Olaf
Hello Olaf,

Sorry to thread hijack. I'm very curious as to how you approach analysis. Following the methylation calls from Bismark, how do you identify differential methylation?

Thanks!

Anthony
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