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Old 10-07-2013, 10:13 PM   #10
bbm
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Location: North Carolina

Join Date: Sep 2011
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Quote:
Originally Posted by Olaf Blue View Post
Hi BBM, basically the way we do the data analysis is (1) to trim off the first 6 bases off of the 5'-end, followed by adapter trimming using "Trimomatic" (I am not a Bioinformatcian so I get this information from my Core facility). After adper sequences are trimmed, then the sequence analysis is performed using Bismark. If you have anything more specific, let me know.

Olaf
Hi Olaf,
Does the strandness matter? I assume the epiGnome kit doesn't produce the stranded library. just curious... BTW: I got the epiGnome kit and testing it out... Do you measure the yield after BS conversion? Did you use 50 ng as the epignome protocol suggested before BS conversion?
Thanks.

Regards,
BBM
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