Hi everybody!
I have a problem with my last run (chip 316) on Ion torrent.
I had only a 31% of loading so I thought it was a charge problem.
But in addition I have 0% of usable reads and 3% of live ISPs.
What's the problem? Could it be the dilution factor of my library (64 barcodes) before the emulsion pcr? or it's an upstream problem when preparing libraries?
Many thanks for who can help me.
Isabella
I have a problem with my last run (chip 316) on Ion torrent.
I had only a 31% of loading so I thought it was a charge problem.
But in addition I have 0% of usable reads and 3% of live ISPs.
What's the problem? Could it be the dilution factor of my library (64 barcodes) before the emulsion pcr? or it's an upstream problem when preparing libraries?
Many thanks for who can help me.
Isabella
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