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Old 11-08-2011, 09:39 AM   #5
TonyBrooks
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Location: London

Join Date: Jun 2009
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Quote:
Originally Posted by Tefina View Post
can you also tell me something about the concentration of the total RNA in the beginning? So assuming that each mRNA has an average length, can you tell me more or less the concentration (nM) in this 50ÁL?
RNA's vary in length so much that it's pointless to estimate a molarity. If you really wanted to, I guess you could do a back of an envelope calculation

According to Ambion M.W. of ssRNA = (# nucleotides x 320.5) + 159.0

Number of moles = Mass (g) / Molecular Weight
Concentration (M) = Number of Moles / Litre

Of which about 2% will be mRNA.
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