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Old 03-18-2015, 01:55 AM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232

It depends on downstream application. For consensus sequence PCR product sequencing would suffice because all possible variants or highly homologous regions will contribute to consensus. To identify SNPs or short indels cloning is preferred. Also for applications such as protein or transgene expression sequencing cloned fragments are necessary to make sure that sequences are error free and in correct frame.
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