Thread: RNase treatment
View Single Post
Old 09-06-2013, 01:13 AM   #5
chma1112860
Junior Member
 
Location: Dijon, France

Join Date: Sep 2013
Posts: 3
Default

Thanks for your answers.

Actually we have about 600 gDNAs already extracted without RNase treatment.
I just wanted to know if it is absolutely necessary to eliminate RNA before starting the library preparation, to avoid useless long work.
We plan to use a Nextera XT kit after making several amplicons of differents sizes.

I think that if we position primers in intronic regions, we won't have amplification artifacts owing to the presence of RNA.
chma1112860 is offline   Reply With Quote