Thread: RNase treatment
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Old 11-01-2010, 01:49 PM   #1
wormseq
Junior Member
 
Location: Florida

Join Date: May 2010
Posts: 2
Default RNase treatment

Hi all,
I have several genomic DNA samples that were extracted using a Qiagen DNeasy kit without the optional RNase treatment that I would like to submit for sequencing (75 bp paired-end libraries for Illumina). I have quantified the samples using a PicoGreen assay and there appears to be plenty of DNA for sequencing. However, I was wondering if there is any reason to be concerned about the residual RNA in the sample interfering somehow with library construction? Any thoughts on this would be greatly appreciated. Thank you.
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