i had a question regarding p5/p7 oligos bound to flow cell and wanted to know which of the following was correct in terms of what is actually bound to the flow cell
1) p5 oligo , p7 oligo (sense or antisense only)
2) p5 oligo, p5 oligo complement, p7 oligo, p7 oligo complement
if scenario 1 is correct, then 1/2 of the libraries do NOT bind to p5 flow cell bound oligo since it'd be in the wrong sense. but then do the other 1/2 (that don't bind to p5) bind to p7 making it so that all library sequences are bound?
1) p5 oligo , p7 oligo (sense or antisense only)
2) p5 oligo, p5 oligo complement, p7 oligo, p7 oligo complement
if scenario 1 is correct, then 1/2 of the libraries do NOT bind to p5 flow cell bound oligo since it'd be in the wrong sense. but then do the other 1/2 (that don't bind to p5) bind to p7 making it so that all library sequences are bound?
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