Hello All,
I have some .bam files from samples of human, two group of sample: First one for Normal and the other for patient, I have no access for the reads files. and i want to generated the genes counts to use it with in edgeR. i am new to these things. Could any one please help me with the steps.
i directly tied the following command after i install the htseq-count
htseq-count -f .bam .gtf > counts.txt
from where i can get the the right .gtf file to use and do i need to put all the .bam files of every group together to get the counts.
Please help
I have some .bam files from samples of human, two group of sample: First one for Normal and the other for patient, I have no access for the reads files. and i want to generated the genes counts to use it with in edgeR. i am new to these things. Could any one please help me with the steps.
i directly tied the following command after i install the htseq-count
htseq-count -f .bam .gtf > counts.txt
from where i can get the the right .gtf file to use and do i need to put all the .bam files of every group together to get the counts.
Please help
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