Hi,
I'm new in the sequencing world (apart from standard courses in university).
I've prepared an RNAseq library using the Scriptseq protocol from Epicentre. Together with a co-worker we sequenced it on a V2 150bp (300 cycles) cassette. Now I'm learning more about sequencing, I'm wondering why we used this cassette and not another one.
Next week, I'm going to prepare a library of small RNA's for sequencing. The fragment size will be around 147 bp (sequence itself + adaptor + indices). But my boss told me to order the v2 cartridge of 50 cycles. Now I'm wondering how long the reads will be that come out of it. If it is 2 x25bp, won't the Miseq read only the adaptor and never the sequences of interest itself?
I'm sorry if these are really silly question, I'm just getting the hang of it.
I'm new in the sequencing world (apart from standard courses in university).
I've prepared an RNAseq library using the Scriptseq protocol from Epicentre. Together with a co-worker we sequenced it on a V2 150bp (300 cycles) cassette. Now I'm learning more about sequencing, I'm wondering why we used this cassette and not another one.
Next week, I'm going to prepare a library of small RNA's for sequencing. The fragment size will be around 147 bp (sequence itself + adaptor + indices). But my boss told me to order the v2 cartridge of 50 cycles. Now I'm wondering how long the reads will be that come out of it. If it is 2 x25bp, won't the Miseq read only the adaptor and never the sequences of interest itself?
I'm sorry if these are really silly question, I'm just getting the hang of it.
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